Ryegrass Fluorescence Test

Biochemical and Molecular Techniques

Utilized by AOSCA Agencies


Fluorescence Test – Ryegrass

This is a test to determine the percentage of ryegrass seedlings with fluorescent roots.  AOSA Rules state that a fluorescence test shall be performed on all samples of ryegrass for which the percentage of perennial ryegrass (Lolium perenne) and/or annual (Italian) ryegrass (Lolium multiflorum) is to be reported.  The seedlings shall be grown on filter paper and the number of fluorescent seedlings determined under ultraviolet light at the end of the germination period.  The AOSA Rules contain example calculations for properly reporting the results.

Labs performing the test: